LL-37 alone and in combination with IL17A enhances proinflammatory cytokine expression in parallel with hyaluronan metabolism in human synovial sarcoma cell line SW982—A step toward understanding the development of inflammatory arthritis
Fig 4
A combination of LL-37 and IL17A induced HA metabolism.
SW982 cells were treated with or without 12.5 ng/mL of LL-37 and/or 5 or 10 ng/mL of IL17A for 6 hr. Cells were then collected and measured for the expression of HAS2, HAS3, HYAL1, HYAL2, CD44 and TLR4 genes, respectively (A-F). SW982 cells were treated with or without 12.5 ng/mL of LL-37 and/or 5 or 10 ng/mL of IL17A for 72 hr before the culture media were collected and measured for HA levels (G). The values from triplicate experiments are expressed as the mean ± SD of fold increases compared to the untreated control. Horizontal brackets indicate significance levels at *p < 0.05 or **p < 0.01.