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General anesthetics cause mitochondrial dysfunction and reduction of intracellular ATP levels

Fig 5

Anesthetic effect on mitochondrial ATP synthesis.

Time course of ATP synthesis by mitochondria with or without anesthetics (n = 3). Mitochondrial ATP synthesis was measured using the MASC assay [21]. Colors are the same as in Fig 4; control (black), 0.3% IF (red), 0.1% 1PP (blue), and 0.05% PBNa (green). We used 10 μg/ml oligomycin as a control inhibitor (purple). Error bars indicate S.D. The results are also summarized in Table 1.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0190213.g005