Mechanisms by which Porphyromonas gingivalis evades innate immunity
Fig 2
Differential effect of P. gingivalis W50 substrains on extinguishing cytokine production from DCs.
(A) DCs from B10.A-Rag2-/- mice were either unstimulated (medium) or stimulated on the sixth day with 5x107 E. coli, F. nucleatum or P. gingivalis alone or a mixture of two bacteria together in a 24-well plate for 16 h at 37°C. CSN were analyzed for the presence of IL-12p75 using a specific ELISA. (B) Same as (A) except that IFNγ was added to the cultures to enhance IL-12 production. P. gingivalis were heat-killed at 60°C for 1 h in a water bath (HKPg), or not, before being added at 5x107/well to the culture containing F. nucleatum or E. coli. (C) DCs were stimulated with 100 ng/ml of LPS (E. coli) plus 100 ng/ml of IFNγ alone (No Pg) or in combination with various strains of P.gingivalis at 5x107/well for 24 h before testing for the presence of IL-12p75 in the CSN. (D) Same as (C) except various numbers of P. gingivalis W50-NIDCR or -ATCC were added to the DCs in a 24-well plate. (A-D) Data are the mean ± SD of compilation of two independent experiments. (D) The data are expressed as the mean ± SD of duplicates.