Mutation in the Pro-Peptide Region of a Cysteine Protease Leads to Altered Activity and Specificity—A Structural and Biochemical Approach
Fig 4
Effect of substitution at residue 86 of pro-peptide of papain on its proteolytic acivity.
a) relative [normalised to WT (TS)] specific activity values against azo-casein and relative Kcat/Km values for the substrate Pyroglutamyl-Phe-Leu-p-nitroanilide. b) The actual values of the same are given in the table. c) Michaelis-Menten plot of proteolytic activity against different substrates for three mutants and WT (TS) papain. The error bar of each points represents standard error of the mean of replicates.