Modulation of Malaria Phenotypes by Pyruvate Kinase (PKLR) Variants in a Thai Population
Fig 4
Effect of R41Q on PKLR protein stability.
(a) Transiently transfected HEK293T cells expressing either the wild-type (WT) or the R41Q mutant PKLR proteins were metabolically labelled with [35S]-methionine followed by chase in label-free media for the indicated time period (in hours). Cell lysates were prepared and PKLR was immunoprecipitated, followed by gel electrophoresis. (b) The radiolabelled PKLR species were quantitated using ImageJ software, and averages from 2–3 independent experiments were calculated as a fraction of the maximum labelling observed at 0 hr.