Subcellular Localization of Matrin 3 Containing Mutations Associated with ALS and Distal Myopathy
Fig 5
Matrin 3 is not a major component of stress granules.
H4 cells were transiently co-transfected with expression plasmids for G3BP1-mCherry (red) and Matrin 3-YFP (WT and F115C). At 24 h post-transfection, one set of cells were treated with sodium arsenite [100μM] for 45 minutes to induce stress granules, which were visualized by the localization of G3BP1-mCherry. WT and F115C Matrin 3 were found to be localized to both the nucleus and cytoplasm when stress granules are present. Untreated H4 cells (-) co-expressing G3BP1-mCherry and Matrin 3-YFP also show both nuclear and cytoplasmic localization. DAPI marks the nucleus (blue). The exposure times for all images in each channel were identical; all of the images in the red channel were exposed for a shorter period than the images in the green channel.