The Sbi Protein Contributes to Staphylococcus aureus Inflammatory Response during Systemic Infection
Fig 4
Induction of inflammatory response by Sbi in vivo.
Groups of BALB/c mice were inoculated by intraperitoneal route with PBS or rSbi (0,075 mg/g of mice). (A and B) Two hours post-inoculation, the levels of IL–6 and TNF-α in plasma were quantified by ELISA and compared with baseline levels. **, P<0,01; ***, P<0,001, Wilcoxon matched–pairs signed rank test. (C and D) Four hours post-inoculation the expression of CXCL-1 and CXCL-10 was determined in cells recovered from peritoneal infiltrates and standardized to GAPDH expression. (E) The percentage of neutrophils recruited to the peritoneum was determined by flow cytometry. Each circle represents a single mouse (A, B and E) or a pool of two mice (C and D) and horizontal lines depict the median for each group. **, P< 0,01; Mann Whitney non parametric test.