Multi-Functional Regulation of 4E-BP Gene Expression by the Ccr4-Not Complex
Fig 5
Regulation of 4E-BP translation by Ccr4-Not complex is not detected.
A. Schematic of reporter constructs that were used for protein level assessment. B. Effect of Not1 reduction on the protein levels of the reporter constructs. Cells were under Not1 RNAi and transfection with the indicated constructs. After serum starvation overnight and insulin stimulation for 30 min, cells were lysed and subjected to Western blotting analysis using antibodies against GFP and tubulin. The GFP protein levels (normalized to tubulin) were densitometrically quantified (Image J) from two independent experiments and normalized to untreated (no RNAi/insulin). Means ± SEM are shown. A representative blot is shown. C. Relative mRNA and protein levels of the reporter constructs normalized to pAAA.