A Highly Functional Synthetic Phage Display Library Containing over 40 Billion Human Antibody Clones
Figure 5
Characterization of the G5 antibody, specific to the alternatively spliced BCD domains of tenascin-C.
(A) Schematic representation of the scFv and SIP antibody format. (B) Biacore characterization of the monomeric fraction of scFv (G5) produced at different concentrations on a CM5 Biacore microsensor chip, coated either with murine (left) or human recombinant BCD domains of tenascin-C (right). (C) SDS-PAGE of the G5 antibody in SIP format in reducing (R) and nonreducing (N) conditions, as well as gel-filtration profile on a S200 10/300 column. (D) Biacore profiles of the G5 antibody in SIP format, against murine and human recombinant BCD domains of tenascin-C.