Cytoplasmic Localization of p21 Protects Trophoblast Giant Cells from DNA Damage Induced Apoptosis
Figure 3
The p21 protein localized to the cytoplasm in TGCs.
After three days of FGF4 deprivation, wild-type and p21−/− TGCs were stained for nuclear DNA (gray), p21 (green) and p57 (red), as in figure 1. The p21−/− TGCs expressed nuclear p57 protein, but not cytoplasmic p21 protein. Images were acquired with an epifluorescence microscope (60x objective). The same exposure time was used for wild-type and p21−/− TGCs acquisitions.