Insulin-Receptor Substrate-2 (IRS-2) Is Required for Maintaining Glucokinase and Glucokinase Regulatory Protein Expression in Mouse Liver
Figure 3
Analysis of GK and GKRP protein expression in liver from IRS-2(−/−), RIP-Irs-2/IRS-2(−/−) and their wild type mice.
Levels of total GK (A) and GKRP (B) were assessed by Western blots of liver lysates prepared from IRS-2(−/−) or from RIP-Irs-2/IRS-2(−/−) and their wild type mice. The bars represent means ± SE of the densitometric values normalized by β-actin (n = 5–8 animals per group). The values obtained in wild type mice were considered as 1. *** p<0.001 (IRS-2(−/−) or RIP-Irs-2/IRS-2(−/−) vs their WT mice). Representative Western blots images are shown.