Insulin-Receptor Substrate-2 (IRS-2) Is Required for Maintaining Glucokinase and Glucokinase Regulatory Protein Expression in Mouse Liver
Figure 1
Glucokinase activity in soluble fractions of liver and hypothalamus.
Extracts were prepared from liver and hypothalamus of female mice of the indicated genotypes. GK activity was calculated by subtracting the glucose-phosphorylating activity at 0.3 mM glucose from the total activity measured at 30 mM glucose. The bars represent means ± SE. n = 8 IRS-2(−/−) (6–7 weeks old), n = 10 WT (6–7 weeks old), n = 5 RIP-Irs-2/IRS-2(−/−) at ∼16 months of age, and n = 5 WT (at ∼16 months of age). GK activity in liver from wild type mice (6–7 weeks old) was considered as 1. *** p<0.001 (IRS-2(−/−) vs WT mice).