Blood-Brain Barrier Breakdown after Embolic Stroke in Rats Occurs without Ultrastructural Evidence for Disrupting Tight Junctions
Figure 2
Expression of claudin-5 and occludin in areas of FITC-albumin extravasation.
Double fluorescence labeling of claudin-5 (blue) and occludin (red), both being transmembrane proteins critical for tight junction formation, demonstrates extravasation of FITC-albumin (green) in the vicinity of vessels expressing both markers. Please also note the presence of discontinuities in the staining pattern of control vessels with an ‘intact’ endothelial barrier (arrow heads).