Loss of Imprinting and Allelic Switching at the DLK1-MEG3 Locus in Human Hepatocellular Carcinoma
Figure 1
Schematic representation of the DLK1-MEG3 imprinted locus on chromosome 14q32.
Maternally expressed genes are depicted in black and paternally expressed genes in grey. The intergenic differentially methylated region (IG-DMR) is located approximately 14 kb upstream to the first exon of MEG3. Studies in patients with UPD chromosome 14 revealed two regions with differential methylation at IG-DMR [30]. Therefore two pyrosequencing assays were developed at this region (IG-DMR1 and 2). In addition, three pyrosequencing assays were designed for one CpG island and two CTCF binding sites within the MEG3-DMR which was found to display parent-of-origin specific methylation (MEG3-DMR1, 2, and 3). Horizontal bar represents scale of 100 kbp.