Zinc Transporter ZIP14 Functions in Hepatic Zinc, Iron and Glucose Homeostasis during the Innate Immune Response (Endotoxemia)
Figure 5
Deletion of Zip14 in mice produces altered glucose homeostasis and IR functions.
(A) Body composition of the WT and Zip14−/− female mice was measured using a NMR Lean/Fat analyzer. (B) Serum and liver glucose from fed-mice were measured by OneTouch UltraMini and colorimetrically, respectively. (C, D) Serum insulin and liver cAMP were measured by ELISA. (E, F) Western analysis results from liver of three mice are shown for each treatment group. (G) Total RNA was isolated from livers and relative transcript abundance for GLUT2, PEPCK, SREBP-1c, FASN and SOCS-3 were measured by qPCR and expressed relative to TBP mRNA as the normalizer. Values are mean ± SE, n = 3−5.