Pericytes Regulate Vascular Basement Membrane Remodeling and Govern Neutrophil Extravasation during Inflammation
Figure 11
Relaxation of pericytes facilitates PMN transmigration in vivo.
(A) Change in area of pericyte gaps in IL-1β-stimulated venular walls exposed to Tolazoline or NE. (B) Change in area of collagen IV LERs in IL-1β-stimulated venules treated with Tolazoline or NE. (C) Change in MFI of collagen IV LERs in IL-1β-stimulated venular walls treated with Tolazoline or NE. (D) Representative images of IL-1β-stimulated cremaster muscle venules illustrating the effect of Tolazoline and NE on PMN transmigration in vivo. The top panels show 2D images from the middle sections of these venules, stained as indicated to reveal transmigrating and extravasated PMNs. Higher magnifications of the boxed areas (marked with Arabic numerals) are displayed on the right. The bottom 3D images are reconstructions of the same venules stained as indicated to reveal extravasated PMNs. (E) Number of PMNs in the process of transmigrating in IL-1β-stimulated cremasters treated with saline, Tolazoline or NE. (F) Number of extravasated PMNs in the perivascular tissues 100 µm around a 200 µm vessel segment in IL-1β-injected mouse cremaster muscles. 7–8 mice and 15 vessel segments from each mouse were analyzed in each group of the experiments displayed. Bar = 10 µm in the zoom-in views, and = 50 µm in other images. ANOVA plus multiple comparisons were performed. Comparisons with saline control were displayed by *P<0.05, **P<0.01, and ***P<0.001. Other comparisons were indicated as ##P<0.01 and ###P<0.001.