Local Oxidative Stress Expansion through Endothelial Cells – A Key Role for Gap Junction Intercellular Communication
Figure 7
JNK activation and nuclear translocation in bystander cells.
bEnd.3 monolayers, were subjected to COI or left untreated and returned into the incubator for 3 more h, then fixed with 4% PFA and stained for gJNK or pJNK (pseudo-colored green) as indicated. After immune-staining, the cell nuclei were counter stained by DAPI (pseudo-colored blue) and imaged by fluorescence microscope. gJNK: Untreated cells exhibit JNK distribution throughout the cell. In treated monolayers, bystander cells adjacent to PI+ cells (marked by arrowheads, 60–80µm away from rim of the COI) show distinct JNK nuclear localization. pJNK: a collage comprised of three snapshots separated by vertical white lines. Bystander cells adjacent to PI+ cells show pJNK in the nucleus (left side) while distant cells have no pJNK staining (right side). Lowest panels are a merge of the upper ones.