Local Oxidative Stress Expansion through Endothelial Cells – A Key Role for Gap Junction Intercellular Communication
Figure 5
Peroxynitrite generation in bEnd.3 bystander cells following COI.
A – Monolayers were subjected to COI, placed back in the incubator for three hours and then subjected to anti-nitrotyrosine antibodies first, and then to fluorescing secondary antibodies (pseudo-colored green). Arrowheads mark dead bystander cells (PI+); blue depicts DAPI nuclear stain. B – Control, as in (A) but with secondary antibodies only, right side arrows mark dead bystander cells. C – Control, untreated monolayers subjected to primary and secondary antibodies. All images represent at least three similar experiments.