Local Oxidative Stress Expansion through Endothelial Cells – A Key Role for Gap Junction Intercellular Communication
Figure 1
Propagation of cell death following COI.
A – bEnd.3 monolayers were subjected to COI. Cell death was monitored by changes in the fluorescence of membrane viability probes, CaAMg and PI, as followed by TLFM. Dead cells are defined as CaAMg negative (green), PI positive (red). Time zero represents termination of COI. B – Dark control (with WST11, no illumination). C – Light control (Illumination, of same rectangle, no WST11). Both control images were taken at 20h. All other details are as described in the Materials and Methods section.