Serratia odorifera a Midgut Inhabitant of Aedes aegypti Mosquito Enhances Its Susceptibility to Dengue-2 Virus
Figure 3
Virus overlay protein binding assay (VOPBA).
Serratia odorifera cell lysate (lanes L1 and L2) were subjected to SDS-PAGE and transferred to Hybond-C membranes. Lane L1 was incubated with 5×105 plaque-forming units of DENV-2, and lane L2 with PBS (pH 7.4) at 37°C. The putative DENV-2 binding proteins were visible after incubation with a rabbit polyclonal antibody to DENV-2 and a secondary antibody (peroxidase-conjugated goat anti-rabbit IgG). The reaction was developed using H2O2 and DABT. The molecular weights of dengue-2 binding proteins are shown on the left side of the blot.