IL-1 and IL-23 Mediate Early IL-17A Production in Pulmonary Inflammation Leading to Late Fibrosis
Figure 1
IL-23p19, IL-17A or IL-17F expression upon BLM or rmIL-1β.
mRNA expression for IL-23p19, Pro-IL-1β and HPRT1 in lung of 4 individual mice treated with BLM (10 mg/kg) or rmIL-1β (50 µg/kg) or saline were assessed using semi-quantitative PCR at 24 h in wild-type mice (A). Semi-quantitative IL-1β expression in the lung, evaluated with IL-1β/HPRT1 PCR ratio, was reduced in absence of IL-23p19 signaling (B). mRNA expression for IL-17A, IL-17F and HPRT1 in lung of 5 individual mice treated with BLM (10 mg/kg) or rmIL-1β (50 µg/kg) or saline wild-type mice or IL-1R1−/− mice were assessed using semi-quantitative PCR at 24 h (C). IL-17A and IL-17F mRNA levels were compared to the housekeeping gene HPRT1 mRNA levels and expressed as IL-17/HPRT ratio by measuring band densities using a densitometric analyzer (ImageJ) (D). Data represent in FiguremRNA expression Data represent mean values ± SD from 3 ratio independent experiments (n = 4 or 5 mice per group; *, p<0.05; **, p<0.01; ***, p<0.001).