Role of Myeloid-Derived Suppressor Cells in Amelioration of Experimental Autoimmune Hepatitis Following Activation of TRPV1 Receptors by Cannabidiol
Figure 8
CBD attenuates SEB-induced acute liver injury by inducing MDSCs in liver.
WT mice (n = 6) were sensitized by injecting D-galactosamine (GalN), 20 mg/mouse in 100 µL PBS (i.p.). After 15 min, SEB was injected (40 µg/mouse, in 100 µL PBS, i.p.). For treatment groups, 10 or 50 mg/kg bd.wt. of CBD was administered i.p. A) Blood was collected at 8 h post SEB injection and serum AST levels were measured. Student's t-test (#p<0.01 compared to Veh/GalN control; *p<0.05, **p<0.01 compared to GalN/SEB). B. Histology: Liver samples after 48 h were fixed and embedded in paraffin. Five µm sections were stained by H&E and analyzed by light microscopy. Representative photomicrographs are shown. a) Veh/GalN, b) CBD50, c) GalN/SEB and d) GalN/SEB+CBD50 (CBD50 = 50 mg/kg). Liver infiltrating cells from the treatment groups as indicated were isolated at 24 h and stained for CD11b and Gr-1 and analyzed by flow cytometry (CBD25 = 25 mg/kg). Representative dot plots are shown with percentages of CD11b+Gr-1+ MDSCs (C). D) Absolute number of MDSCs in liver for each group (n = 4). Student's t-test (**p<0.01 compared to GalN/SEB).