An Anti-HIV-1 V3 Loop Antibody Fully Protects Cross-Clade and Elicits T-Cell Immunity in Macaques Mucosally Challenged with an R5 Clade C SHIV
Figure 3
Induction of specific antiviral T-cell responses after passive immunization.
(A) and (B) Proliferation of T cells. PBMC were stimulated with SIVmac251 Gag protein and proliferation of CD4+ and CD8+ cells was measured using the CFSE dilution method. (A) Animals given 50 mg/kg of HGN194 (Group 1A) and control group RM (Group 2). PBMC collected 17 weeks post-challenge were tested. Horizontal dashed line, cut-off value based on response shown by a naïve animal. (B) Animals given 1 mg/kg of HGN194 (Group 1B). PBMC collected at weeks 12 (RNc-13) and 17 (ROa-13) post-challenge were tested. (C) Absolute numbers of CD8+ T cells for Groups 1A (black) and 1B (grey) after treatment with a cytotoxic anti-CD8 mAb (Methods). (D) Plasma viral RNA load after CD8+ cell depletion.