Matched Sizes of Activating and Inhibitory Receptor/Ligand Pairs Are Required for Optimal Signal Integration by Human Natural Killer Cells
Figure 5
Size differences drive segregation of different length variants of HLA-Cw6 within immune synapses.
221 cells double-transfected with normal-length or CD4-elongated forms of Cw6 fused to either GFP (green) or mCherry (red) were incubated for 30 min with KIR2DL1-expressing YTS transfectants and conjugates were imaged by live-cell confocal microscopy. En-face views of the synapse were reconstructed and the Pearson correlation coefficient (r) for GFP and mCherry fluorescence were determined. Mean ± SD of the total number of conjugates is shown. The scale bar represents 10 µm.