Cpd-1 Null Mice Display a Subtle Neurological Phenotype
Figure 1
A. Loss of CPD1 message in CPD1 null mice. RNA extracted from brains of CPD1 +/+, CPD1 +/−, and CPD−/− mice show loss of CPD1 transcripts by RT-PCR analysis. GAPDH is used as an internal control. Reduction in CPD1 transcript level is evident in CPD1 +/− mice. B. Antibody to CPD1 does not cross-react against ANP homologues. FLAG-tagged versions of CPD1, LANP and APRIL were transfected into cells and immunoprecipitaed with anti-FLAG antibody. The antibody to CPD1 specifically recognizes CPD1 and not the other ANP homologues. Western blot with FLAG demonstrates the transfection and pull-down of the relevant proteins. C. CPD1 null mice do not express CPD1 protein. Protein extracted from brains of CPD1 +/+, CPD1 +/−, and CPD−/− mice show levels of CPD1 protein by western blot analysis. Actin is used as loading control. Asterisks denote non-specific bands. D. Expression of endogenous CPD1. Immunostaining of neuro2a cells with α-CPD1 antibody displays localization of CPD1 protein. DAPI staining marks the nucleus. E. Immunostaining on brains dissected from CPD1 +/+ and CPD1 −/− mice shows reduced CPD1 staining in CPD1 null mice.