Inhibition of the Progesterone Nuclear Receptor during the Bone Linear Growth Phase Increases Peak Bone Mass in Female Mice
Figure 5
Osteoblast differentiation and apoptosis-related gene expressions in osteoblastic cultures.
Bone marrow cells were extracted from 3-month-old mice and cultured with ascorbic acid and β-glycerophosphate from WT and PRKO mice to monitor osteoblastogenesis. RNA was extracted from the cultures on day 14. Real-time PCR was performed to monitor gene expression for osteoblast differentiation (Runx2, Osterix, Osteocalcin, OPG and RANKL) or apoptosis (Foxo1 and FasL). *p<0.05 compared with WT of the same sex.