Phosphorylation of Human Tristetraprolin in Response to Its Interaction with the Cbl Interacting Protein CIN85
Figure 5
Localization of hTTP and MEKK4 with CIN85 in cultured cells.
In A, HEK 293 cells were transfected with plasmids for the expression of HA-hTTP (red) and Flag-CIN85 (Green), or (B) HA-MEKK4 (red) and Flag-CIN85 (green). The cells were stained with primary antibodies, either an anti-HA polyclonal or anti-Flag monoclonal, followed by the secondary antibodies Alexa 594 anti-rabbit (red) or Alexa 488 anti-mouse (green), respectively. Nuclei (blue) were stained with DAPI. The cells were visualized and images obtained by confocal microscopy. The merged images of two protein signals, indicating areas of apparent co-localization, are shown in yellow.