Neutrophils Contribute to the Protection Conferred by ArtinM against Intracellular Pathogens: A Study on Leishmania major
Fig 5
ArtinM augments ROS production by L. major-infected neutrophils.
Human neutrophils were treated with PMA, fMLP, ArtinM or medium (untreated). ROS production was quantified by reaction with luminol (A) and lucigenin (B), producing chemiluminescent photons (CPS). Data on PMA stimulation or L. major promastigotes infected neutrophils (3:1), which were treated or not treated with ArtinM, are shown in panel C. The amounts of ROS released are expressed as mean AUCxCPS ± SD. ** p<0.01; *** p<0.001; **** p<0.0001. One way ANOVA followed by Bonferroni's post-test. Each assay was carried out in triplicate. The data shown are representative of three independent experiments.