Cohesin Protects Genes against γH2AX Induced by DNA Double-Strand Breaks
Figure 5
Cohesin is required to ensure transcriptional maintenance in γH2AX domains after 4OHT treatment.
RNAs were prepared and reverse transcribed from AsiSI-ER-U20S cells transfected with control or SCC1 siRNA before and after 4 hours of 4OHT treatment (as indicated). cDNA levels for several genes encompassed in γH2AX domains were measured by Quantitative PCR. Data were normalized against P0 (ribosomal protein) cDNA. Data were expressed as cDNA fold change between 4OHT treated and untreated cells. The mean and SDOM from five independent experiments are shown. The p value above each bar indicates one sample t-test against a theoretical ratio of 1 (no change between +4OHT and −4OHT) (* p<0.1, ** p<0.05, *** p<0.01).