Cohesin Protects Genes against γH2AX Induced by DNA Double-Strand Breaks
Figure 3
Cohesin depletion leads to an increase of γH2AX.
A, γH2AX ChIP-chip were performed after 4OHT treatment in control (black) or SCC1 depleted (red) cells. The log2 γH2AX/input signal from two independent experiments was averaged around the 24 digested AsiSI sites from chromosomes 1 and 6 (see Table S1) (left panel), or on averaged around 24 random sites located outside γH2AX domains (right panel). B, Detailed views of the ChIP-chip signals (from two experiments) obtained for SMC3 (black), γH2AX in control cells (red) and γH2AX in cells treated with SCC1 siRNA (orange), on three cohesin-bound regions (upper and middle panels) and one cohesin-unbound region (lower panel). C, The averaged SMC3 signal over an 80 kb window around each AsiSI site was calculated (x-axis) and plotted against the γH2AX ratio in cells transfected with siRNA_SCC1 versus siRNA_CTRL (y axis). The correlation coefficient and the p value are indicated.