GTPase Activity Plays a Key Role in the Pathobiology of LRRK2
Figure 4
LRRK2 GTPase variants induce defects in autophagy.
(A) Transmission electron micrographs demonstrating increased autophagic vacuoles (asterix) within the vacuole of yeast cells expressing the toxic GTPase variants, K1347A or T1348N, in the LRRK2 GTP-COR-Kin fragment. V, vacuoles; N, nucleus; Scale bars, 2 µm. (B) Quantification of autophagic defects showing the percentage of cells with autophagic vacuoles. A total of 100 cells were analyzed in one experiment and data are representative of at least two independent experiments. Bars represent the mean ± SEM. Data were analyzed for statistical significance by two-tailed unpaired Student's t-test relative to WT LRRK2, or by pair-wise comparisons with vector controls where indicated by horizontal lines (*P<0.01 and **P<0.001). n.s., non-significant versus WT.