Caenorhabditis elegans HIM-18/SLX-4 Interacts with SLX-1 and XPF-1 and Maintains Genomic Integrity in the Germline by Processing Recombination Intermediates
Figure 2
HIM-18 is dispensable for homologous pairing and synapsis.
(A, B) Homologous pairing and synapsis were assayed by FISH and immunostaining on squashed mid-pachytene nuclei from wild type and him-18 mutants. The synaptonemal complex was visualized with the α−SYP-1 antibody and pairing was assessed with a FISH probe targeting the 5S rDNA locus on chromosome V. SYP-1 localizes at the interface between aligned DAPI-stained chromosome pairs. The single or closely juxtaposed hybridization signals observed on each nucleus (yellow arrows) indicate homologous pairing. (C, D) Co-immunostaining with α-SYP-1 and α-HIM-8 to assess synapsis and pairing at the pairing center end of chromosome X in mid-pachytene nuclei. Arrows indicate the single HIM-8 focus observed on each nucleus. (E) Illustration depicting homologous pairing and synapsis. Bar, 2 µm.