Phosphoinositide Metabolism Links cGMP-Dependent Protein Kinase G to Essential Ca2+ Signals at Key Decision Points in the Life Cycle of Malaria Parasites
Figure 6
PKG controls phosphoinositide metabolism and Ca2+ mobilisation in P. falciparum schizonts prior to merozoite egress.
(A) Relative fluorescence intensity of ∼108 Fluo-4–loaded synchronised P. falciparum schizonts in response to simultaneous exposure to 100 µM zaprinast and increasing concentrations of C2. Data are representative of two independent experiments. (B) Relative abundance over time of PI, PIP (left panel), PIP2, and PIP3 (right panel, note different scale) after simultaneous inhibition of PDEs by zaprinast and PKG by C2. The response of P. falciparum 3D7 (dashed lines) is compared to a transgenic clone expressing the C2-resistant PKGT618Q allele (solid lines).