Posttranscriptional Gene Regulation by Spatial Rearrangement of the 3′ Untranslated Region
Figure 3
The Distance between the PTC and the Poly(A) Tail Is an Important Criterion for mRNA Stability
(A) Schematic illustration of the miniμ C3/H4 ter440 FB mRNAs expressed by the indicated constructs. Complementary sequences to the regions FB, FB2, FB3, FB4, and FB5 were inserted as double-stranded oligonucleotides at the position marked by the arrow. The distance (in number of nucleotides) between the PTC (ter440) and the first base of the respective base pairing region are indicated below.
(B) Half-lives of the FB mRNAs were measured as described in Figure 1C. Average values and SD from one experiment with three RT-qPCR runs are shown.
(C) Half-lives of the indicated mRNAs (data from Figures 2B and 3B) were plotted against the distance between ter440 and the first base of the base-pairing region.