Abstract

We have developed a wide-field total-internal-reflection fluorescence microscope capable of imaging single molecules in live cells, resolved in both wavelength and polarization. We show fluorescence resonance energy transfer between single pairs of fluorescent molecules bound to signaling receptors in the plasma membrane of live cells and demonstrate the importance of polarization discrimination in addition to wavelength separation.

© 2006 Optical Society of America

Enhanced live cell membrane imaging using surface plasmon-enhanced total internal reflection fluorescence microscopy

Ruei-Yu He, Guan-Liang Chang, Hua-Lin Wu, Chi-Hung Lin, Kuo-Chih Chiu, Yuan-Deng Su, and Shean-Jen Chen
Opt. Express 14(20) 9307-9316 (2006)

Extended resolution wide-field optical imaging: objective-launched standing-wave total internal reflection fluorescence microscopy

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Simultaneous widefield single molecule orientation and FRET microscopy in cells

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Thin-film-based sensitivity enhancement for total internal reflection fluorescence live-cell imaging

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Diagnosis of colorectal cancer using Raman spectroscopy of laser-trapped single living epithelial cells

Kun Chen, Yejun Qin, Feng Zheng, Menghong Sun, and Daren Shi
Opt. Lett. 31(13) 2015-2017 (2006)