Abstract

We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [Cuche, et al., Appl. Opt. 38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques.

© 2005 Optical Society of America

Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy

Benjamin Rappaz, Pierre Marquet, Etienne Cuche, Yves Emery, Christian Depeursinge, and Pierre J. Magistretti
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High-resolution quantitative phase-contrast microscopy by digital holography

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Optofluidic bioimaging platform for quantitative phase imaging of lab on a chip devices using digital holographic microscopy

Vimal Prabhu Pandiyan and Renu John
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