Expression of Pkd2l2 in Testis Is Implicated in Spermatogenesis

Ye Chen; Zheng Zhang; Xiao-Yan Lv; Yi-Dong Wang; Zhong-Guo Hu; Huan Sun; Rui-Zhi Tan; Yu-Hang Liu; Guo-Hui Bian; Yan Xiao; Qin-Wei Li; Qiu-Tan Yang; Jian-Zhong Ai; Lu Feng; Yang Yang; Yu-Quan Wei; Qin Zhou

doi:10.1248/bpb.31.1496

Regular Articles

Expression of Pkd2l2 in Testis Is Implicated in Spermatogenesis

Ye Chen, Zheng Zhang, Xiao-Yan Lv, Yi-Dong Wang, Zhong-Guo Hu, Huan Sun, Rui-Zhi Tan, Yu-Hang Liu, Guo-Hui Bian, Yan Xiao, Qin-Wei Li, Qiu-Tan Yang, Jian-Zhong Ai, Lu Feng, Yang Yang, Yu-Quan Wei, Qin Zhou

Author information

Ye Chen
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Zheng Zhang
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Xiao-Yan Lv
Department of Dermatology, West China Hospital, Sichuan University
Yi-Dong Wang
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Zhong-Guo Hu
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Huan Sun
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Rui-Zhi Tan
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Yu-Hang Liu
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Guo-Hui Bian
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Yan Xiao
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Qin-Wei Li
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Qiu-Tan Yang
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Jian-Zhong Ai
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Lu Feng
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Yang Yang
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Yu-Quan Wei
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University
Qin Zhou
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University

Keywords: Pkd2l2, polycystic kidney disease, testis, spermatogenesis, intracellular calcium

JOURNAL FREE ACCESS

2008 Volume 31 Issue 8 Pages 1496-1500

DOI https://doi.org/10.1248/bpb.31.1496

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Abstract

Pkd2l2 is a novel member of the polycystic kidney disease (PKD) gene family in mammals. Prominently expressed in testis, this gene is still poorly understood. In this study, reverse transcription polymerase chain reaction (RT-PCR) results showed a time-dependent expression pattern of Pkd2l2 in postnatal mouse testis. Immunohistochemical analysis revealed that Pkd2l2 encoded a protein, polycystin-L2, which was predominantly detectable in the plasma membrane of spermatocytes and round spermatids, as well as in the head and tail of elongating spermatids within seminiferous tubules in mouse testis tissue sections of postnatal day 14 and adult mice. A green fluorescent fusion protein of Pkd2l2 resided in the plasma membrane of HEK 293 and MDCK cells, suggesting that it functions as a plasma membrane protein. Overexpression of Pkd2l2 increased the intracellular calcium concentration of MDCK cells, as detected by flow cytometry. Collectively, these data indicated that Pkd2l2 may be involved in the mid-late stage of spermatogenesis through modulation of the intracellular calcium concentration.

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