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Cap-independent translation initiation of Apaf-1 mRNA based on a scanning mechanism is determined by some features of the secondary structure of its 5′ untranslated region

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Abstract

We have earlier shown that the 5′-untranslated region (5′ UTR) of the mRNA coding for activation factor of apoptotic peptidase 1 (Apaf-1) can direct translation in vivo by strictly 5′ end-dependent way even in the absence of m7G-cap. Dependence of translational efficiency on the cap availability for this mRNA turned out to be relatively low. In this study we demonstrate that this surprising phenomenon is determined the 5′-proximal part (domains I and II) of highly structured Apaf-1 5′ UTR. Remarkably, domain II by itself was able to reduce dependence of the mRNA on the cap on its transferring to a short 5′ UTR derived from a standard vector. We suggest that the low cap-dependence inherent to some cellular mRNAs may have an important physiological significance under those stress conditions when the function of cap-binding factor eIF4E is impaired.

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Abbreviations

Apaf-1:

apoptotic peptidase activating factor 1

CITEs:

cap-independent translational enhancers

eIF:

eukaryotic initiation factor

IRES:

internal ribosome entry site

5′ UTR:

5′-untranslated region

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Authors and Affiliations

  1. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia

    D. E. Andreev, S. E. Dmitriev, I. M. Terenin & I. N. Shatsky

Authors
  1. D. E. Andreev
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  2. S. E. Dmitriev
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  3. I. M. Terenin
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  4. I. N. Shatsky
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Corresponding author

Correspondence to I. N. Shatsky.

Additional information

Published in Russian in Biokhimiya, 2013, Vol. 78, No. 2, pp. 220–229.

Originally published in Biochemistry (Moscow) On-Line Papers in Press as Manuscript BM12-254, January 13, 2013.

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Andreev, D.E., Dmitriev, S.E., Terenin, I.M. et al. Cap-independent translation initiation of Apaf-1 mRNA based on a scanning mechanism is determined by some features of the secondary structure of its 5′ untranslated region. Biochemistry Moscow 78, 157–165 (2013). https://doi.org/10.1134/S0006297913020041

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  • DOI: https://doi.org/10.1134/S0006297913020041

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