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The nucleoporin 153, a novel factor in double-strand break repair and DNA damage response

Oncogene volume 31, pages 4803–4809 (2012)Cite this article

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Abstract

DNA repair is essential in maintaining genome integrity and defects in different steps of the process have been linked to cancer and aging. It is a long lasting question how DNA repair is spatially and temporarily organized in the highly compartmentalized nucleus and whether the diverse nuclear compartments regulate differently the efficiency of repair. Increasing evidence suggest the involvement of nuclear pore complexes in repair of double-strand breaks (DSBs) in yeast. Here, we show that the human nucleoporin 153 (NUP153) has a role in repair of DSBs and in the activation of DNA damage checkpoints. We explore the mechanism of action of NUP153 and we propose its potential as a novel therapeutic target in cancers.

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Acknowledgements

We thank Amélie Weiss, Laure Froidevaux and Audrey Furst for excellent technical assistance. The GFP-NUP153 vector was provided by Ian Ellenberg (EMBL, Heidelberg). We are grateful to Zita Nagy and Tibor Pankotai for critical reading of the manuscript. C.L. is supported by the Région Alsace. The research in E.S. lab is funded by CNRS, ANR, INCA and HFSP. We thank the imaging center of IGBMC and particularly Marc Koch for the help with the multi photon laser experiments.

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Authors and Affiliations

  1. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), UMR 7104 CNRS, CU de Strasbourg, France

    C Lemaître, A Kalousi, A-S Hoffbeck & E Soutoglou

  2. High Throughput Screening Facility, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), UMR 7104 CNRS, CU de Strasbourg, France

    B Fischer & L Brino

  3. CNRS, UMR217, Institute for Cellular and Molecular Radiobiology, Fontenay aux Roses, France

    J Guirouilh-Barbat, D Genet, P Betrand & B Lopez

  4. Department of Genetics, The Institute of Life Sciences, The Hebrew University, Jerusalem, Israel

    O D Shahar & M Goldberg

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  1. C Lemaître
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  2. B Fischer
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  3. A Kalousi
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  4. A-S Hoffbeck
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  5. J Guirouilh-Barbat
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  7. D Genet
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  8. M Goldberg
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  9. P Betrand
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  10. B Lopez
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  11. L Brino
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  12. E Soutoglou
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Correspondence to E Soutoglou.

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Supplementary Information accompanies the paper on the Oncogene website

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Lemaître, C., Fischer, B., Kalousi, A. et al. The nucleoporin 153, a novel factor in double-strand break repair and DNA damage response. Oncogene 31, 4803–4809 (2012). https://doi.org/10.1038/onc.2011.638

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  • DOI: https://doi.org/10.1038/onc.2011.638

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