Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Brief Communication
  • Published:

The stalk region of dynamin drives the constriction of dynamin tubes

Nature Structural & Molecular Biology volume 11, pages 574–575 (2004)Cite this article

Abstract

The GTPase dynamin is essential for numerous vesiculation events including clathrin-mediated endocytosis. Upon GTP hydrolysis, dynamin constricts a lipid bilayer. Previously, a three-dimensional structure of mutant dynamin in the constricted state was determined by helical reconstruction methods. We solved the nonconstricted state by a single-particle approach and show that the stalk region of dynamin undergoes a large conformational change that drives tube constriction.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Image analysis of dynamin lipid tubes.
Figure 2: Comparison of ΔPRD dynamin lipid tubes in nonconstricted and constricted states.

Similar content being viewed by others

References

  1. Hinshaw, J.E. Annu. Rev. Cell Dev. Biol. 16, 483–519 (2000).

    Article  CAS  Google Scholar 

  2. Danino, D. & Hinshaw, J.E. Curr. Opin. Cell Biol. 13, 454–460 (2001).

    Article  CAS  Google Scholar 

  3. Song, B.D. & Schmid, S.L. Biochemistry 42, 1369–1376 (2003).

    Article  CAS  Google Scholar 

  4. Smirnova, E., Shurland, D.L., Newman-Smith, E.D., Pishvaee, B. & van der Bliek, A.M. J. Biol. Chem. 274, 14942–14947 (1999).

    Article  CAS  Google Scholar 

  5. Sweitzer, S.M. & Hinshaw, J.E. Cell 93, 1021–1029 (1998).

    Article  CAS  Google Scholar 

  6. Zhang, P. & Hinshaw, J.E. Nat. Cell Biol. 3, 922–926 (2001).

    Article  CAS  Google Scholar 

  7. Danino, D., Moon, K. & Hinshaw, J.E. J. Struct. Biol. in the press.

  8. Egelman, E.H. Ultramicroscopy 85, 225–234 (2000).

    Article  CAS  Google Scholar 

  9. Okamoto, P.M., Tripet, B., Litowski, J., Hodges, R.S. & Vallee, R.B. J. Biol. Chem. 274, 10277–10286 (1999).

    Article  CAS  Google Scholar 

  10. Eccleston, J.F., Binns, D.D., Davis, C.T., Albanesi, J.P. & Jameson, D.M. Eur. Biophys. J. 31, 275–282 (2002).

    Article  CAS  Google Scholar 

  11. Warnock, D.E., Hinshaw, J.E. & Schmid, S.L. J. Biol. Chem. 271, 22310–22314 (1996).

    Article  CAS  Google Scholar 

  12. Stowell, M.H., Marks, B., Wigge, P. & McMahon, H.T. Nat. Cell Biol. 1, 27–32 (1999).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank K.-H. Moon for his help with the helical hand determination. This work was supported by a grant from the US National Institute of Biomedical Imaging and Bioengineering, US National Institutes of Health, to E.H.E.

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, Virginia, USA

    Yen-Ju Chen & Edward H Egelman

  2. Laboratory of Cell Biochemistry and Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA

    Peijun Zhang & Jenny E Hinshaw

Authors
  1. Yen-Ju Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Peijun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Edward H Egelman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jenny E Hinshaw
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Edward H Egelman.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, YJ., Zhang, P., Egelman, E. et al. The stalk region of dynamin drives the constriction of dynamin tubes. Nat Struct Mol Biol 11, 574–575 (2004). https://doi.org/10.1038/nsmb762

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsmb762

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing