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Role of the MEOX2 homeobox gene in neurovascular dysfunction in Alzheimer disease

Nature Medicine volume 11, pages 959–965 (2005)Cite this article

Abstract

Neurovascular dysfunction substantially contributes to Alzheimer disease. Here, we show that transcriptional profiling of human brain endothelial cells (BECs) defines a subset of genes whose expression is age-independent but is considerably altered in Alzheimer disease, including the homeobox gene MEOX2 (also known as GAX), a regulator of vascular differentiation, whose expression is low in Alzheimer disease. By using viral-mediated MEOX2 gene silencing and transfer, we show that restoring expression of the protein it encodes, GAX, in BECs from individuals with Alzheimer disease stimulates angiogenesis, transcriptionally suppresses AFX1 forkhead transcription factor–mediated apoptosis and increases the levels of a major amyloid-β peptide (Aβ) clearance receptor, the low-density lipoprotein receptor–related protein 1 (LRP), at the blood-brain barrier. In mice, deletion of Meox2 (also known as Gax) results in reductions in brain capillary density and resting cerebral blood flow, loss of the angiogenic response to hypoxia in the brain and an impaired Aβ efflux from brain caused by reduced LRP levels. The link of MEOX2 to neurovascular dysfunction in Alzheimer disease provides new mechanistic and therapeutic insights into this illness.

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Figure 1: Gene expression and function of Alzheimer disease neurovascular cells.
Figure 2: MEOX2 homeobox gene determines Alzheimer disease–like phenotype in neurovascular cells.
Figure 3: Meox2 gene deletion results in cerebrovascular incompetence in mice.
Figure 4: Meox2+/− mice show impaired Aβ clearance and LRP downregulation.
Figure 5: GAX-mediated regulation of LRP in primary human BECs.

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Acknowledgements

This work was supported by US National Institutes of Health (NIH) R37 AG023084 and a Socratech research grant to B.V.Z., NIH grants R43 AG24002 to J.S. and R43 AG23993 to N.C.

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Author notes

  1. Zhenhua Wu, Huang Guo, Nienwen Chow and Jan Sallstrom: These authors contributed equally to this work.

Authors and Affiliations

  1. Frank P. Smith Laboratories for Neuroscience and Neurosurgical Research, University of Rochester Medical Center, Arthur Kornberg Medical Research Building, 601 Elmwood Avenue, Box 670, Rochester, 14642, New York, USA

    Zhenhua Wu, Huang Guo, Rashid Deane, Abhay Sagare, Dong Liu, Xiaomei Song & Berislav V Zlokovic

  2. Socratech Research Laboratories, 625 Elmwood Avenue, Rochester, 14620, New York, USA

    Huang Guo, Nienwen Chow, Jan Sallstrom, Robert D Bell, Suhasini Kanagala, Anna Rubio, Fang Li, Don Armstrong, Raphael Zidovetzki, Florence Hofman & Berislav V Zlokovic

  3. Genomics Center, University of Rochester Medical Center, 575 Elmwood Avenue, Box EHSC, Rochester, 14642, New York, USA

    Andrew I Brooks

  4. Department of Cell Biology and Neuroscience, University of California Riverside, 1208 Spieth Hall, Riverside, 92521, California, USA

    Don Armstrong & Raphael Zidovetzki

  5. Department of Environmental Medicine, University of Rochester School of Medicine & Dentistry, Box EHSC, 575 Elmwood Avenue, Rochester, 14642, New York, USA

    Thomas Gasiewicz

  6. Department of Pathology, Keck School of Medicine, University of Southern California, Hoffman Medical Research Center 209, 2011 Zonal Avenue, Los Angeles, 90089-9092, California, USA

    Florence Hofman

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Correspondence to Berislav V Zlokovic.

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Competing interests

J. Sallstrom, N. Chow and R. Bell are Socratech employees, and B.V. Zlokovic, F. Hofman and R. Zidovetzki serve as Socratech consultants. Socratech LLS sponsored transcriptional profiling study and building of the BioBank with primary neurovascular cells. Socratech has an interest in developing new theories for Alzheimer's disease.

Supplementary information

Supplementary Fig. 1

TUNEL-positive and AFX1-positive microvessels in Alzheimer disease and age-matched control brains. (PDF 8635 kb)

Supplementary Fig. 2

Vascular reactivity in Gax+/+ and Gax+/− mice. (PDF 533 kb)

Supplementary Fig. 3

Brain capillary length and LRP levels in Ahr−/− mice. (PDF 1021 kb)

Supplementary Fig. 4

Internalization of α2M in BECs with suppressed MEOX2 expression. (PDF 327 kb)

Supplementary Fig. 5

Proteasomal proteolytic activity, transferrin levels and RAP in human BECs. (PDF 422 kb)

Supplementary Fig. 6

Expression of MEF2, ankyrin G, plectin 1 and TINUR in human BECs. (PDF 513 kb)

Supplementary Fig. 7

Aβ does not affect GAX expression in human BECs (PDF 491 kb)

Supplementary Table 1 (PDF 61 kb)

Supplementary Table 2 (PDF 94 kb)

Supplementary Methods (PDF 64 kb)

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Wu, Z., Guo, H., Chow, N. et al. Role of the MEOX2 homeobox gene in neurovascular dysfunction in Alzheimer disease. Nat Med 11, 959–965 (2005). https://doi.org/10.1038/nm1287

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