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Deregulation of cell growth by the K1 gene of Karposi's sarcoma-associated herpesvirus

Nature Medicine volume 4, pages 435–440 (1998)Cite this article

Abstract

At a position equivalent to the gene encoding the saimiri transforming protein (STP) of herpesvirus saimiri (HVS), Kaposi's sarcoma-associated herpesvirus (KSHV) contains a distinct open reading frame called K1. Although KSHV and HVS are related members of the rhadinovirus subgroup of gamma herpesviruses, K1 and STP exhibit no similarity in amino acid sequence or in structural organization. Since STP is required for the oncogenic potential of HVS, we investigated the functional consequence of K1 expression. Expression of the K1 gene in rodent fibrob-lasts produced morphologic changes and focus formation indicative of transformation. A recombinant herpesvirus in which the STP oncogene of HVS was replaced with K1, immortalized primary T lymphocytes to IL-2 independent growth and induced lymphoma in common marmosets. These results demonstrate the transforming potential of the K1 gene of KSHV.

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Authors and Affiliations

  1. New England Regional Primate Research Center, Harvard Medical School, 1 Pine Hill Drive, Southborough, MA, 01772-9102, USA

    Heuiran Lee, Ronald Veazey, Kenneth Williams, Mengtao Li, Jie Guo, Andrew Lackner, Ronald C. Desrosiers & Jae U. Jung

  2. Institute fur Klinishe und Molekulare Virologies, Friedrich-Alexander Universitat, Loschgestrasse 7, D-8520, Erlangen, Germany

    Frank Neipel & Bernhard Fleckenstein

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Lee, H., Veazey, R., Williams, K. et al. Deregulation of cell growth by the K1 gene of Karposi's sarcoma-associated herpesvirus. Nat Med 4, 435–440 (1998). https://doi.org/10.1038/nm0498-435

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