Abstract
DNA polymerase κ (pol κ) is a member of the Y-family of DNA polymerases that are thought to function in translesion synthesis (TLS) past different types of DNA damage. Here, we show that pol κ-deficient mouse cells have substantially reduced (but not absent) levels of nucleotide excision repair (NER) of UV damage, as measured by several methods. Our results provide evidence for an unexpected role for pol κ in mammalian NER.
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References
Avkin, S. et al. J. Biol. Chem. 279, 53298–58305 (2004).
Ogi, T., Shinkai, Y., Tanaka, K. & Ohmori, H. Proc. Natl Acad. Sci. USA 99, 15548–15553. (2002).
Ogi, T., Kannouche, P. & Lehmann, A. R. J. Cell Sci. 118, 129–136 (2005).
Bi, X., Slater, D. M., Ohmori, H. & Vaziri, C. J. Biol. Chem. 280, 22343–22355 (2005).
Okada, T. et al. J. Biol. Chem. 277, 48690–48695 (2002).
Ohashi, E. et al. Genes Dev. 14, 1589–1594 (2000).
Shivji, M., Podust, V. N., Hubscher, U. & Wood, R. D. Biochemistry 34, 5011–5017 (1995).
Syvaoja, J. et al. Proc. Natl Acad. Sci. USA 87, 6664–6668 (1990).
Dresler, S. L. J. Biol. Chem. 259, 13947–13952 (1984).
Einolf, H. J. & Guengerich, F. P. J. Biol. Chem. 275, 16316–16322 (2000).
Suzuki, N. et al. Biochemistry 40, 15176–15183 (2001).
Haracska, L., Prakash, L. & Prakash, S. Proc. Natl Acad. Sci. USA 99, 16000–16005 (2002).
Godoy, V. G., Jarosz, D. F., Walker, F. L., Simmons, L. A. & Walker, G. C. EMBO J. 25, 868–879 (2006).
Kawamoto, T. et al. Mol. Cell 20, 793–799 (2005).
McIlwraith, M. J. et al. Mol. Cell 20, 783–792 (2005).
Acknowledgements
We are grateful to H. Ohmori for helpful comments and for the Polk-deficient MEFs, and to H. Fawcett and L. Ju for technical assistance. This work was supported by a Medical Research Council (MRC) programme grant to A.R.L. and an Uehara Memorial Foundation grant to T.O.
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Ogi, T., Lehmann, A. The Y-family DNA polymerase κ (pol κ) functions in mammalian nucleotide-excision repair. Nat Cell Biol 8, 640–642 (2006). https://doi.org/10.1038/ncb1417
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DOI: https://doi.org/10.1038/ncb1417
