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CtBP3/BARS drives membrane fission in dynamin-independent transport pathways

Nature Cell Biology volume 7, pages 570–580 (2005)Cite this article

An Addendum to this article was published on 01 October 2005

A Corrigendum to this article was published on 01 June 2005

Abstract

Membrane fission is a fundamental step in membrane transport. So far, the only fission protein machinery that has been implicated in in vivo transport involves dynamin, and functions in several, but not all, transport pathways. Thus, other fission machineries may exist. Here, we report that carboxy-terminal binding protein 3/brefeldin A-ribosylated substrate (CtBP3/BARS) controls fission in basolateral transport from the Golgi to the plasma membrane and in fluid-phase endocytosis, whereas dynamin is not involved in these steps. Conversely, CtBP3/BARS protein is inactive in apical transport to the plasma membrane and in receptor-mediated endocytosis, both steps being controlled by dynamin. This indicates that CtBP3/BARS controls membrane fission in endocytic and exocytic transport pathways, distinct from those that require dynamin.

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Figure 1: Effects of BARS microinjection on post-Golgi transport of VSVG in COS7 cells.
Figure 2: Characterization of the effects of BARS on post-Golgi VSVG transport.
Figure 3: Effects of BARS inhibition on post-Golgi transport of VSVG.
Figure 4: The roles of BARS and dynamin in post-Golgi carrier formation.
Figure 5: Differential roles of BARS and dynamin in the export of VSVG and p75 from the TGN.
Figure 6: Differential requirements for BARS and dynamin in transferrin and fluid-phase endocytosis.

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Acknowledgements

The authors would like to thank M. A. De Matteis for critical reading of the manuscript; M. McNiven, M. A. De Matteis and S. Ponnambalam for antibodies; F. Mirabella for supplying constructs; C. P. Berrie for editorial assistance; and the Italian Association for Cancer Research (AIRC, Milan, Italy), Telethon Italia, and the Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR, Italy) for financial support. M.B. and S.S. are fellows of the Italian Foundation for Cancer Research (FIRC, Milan, Italy). This paper is dedicated to the memory of Julius Axelrod.

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Author notes

  1. Matteo Bonazzi and Stefania Spanò: These authors contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Membrane Traffic, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), 66030, Italy

    Matteo Bonazzi, Gabriele Turacchio, Hee Seok Kweon, Elena V. Polishchuck, Roman S. Polishchuck, Michele Sallese, Teodoro Pulvirenti & Alberto Luini

  2. Department of Cell Biology and Oncology, Laboratory of Cell Regulation, Consorzio Mario Negri Sud, Santa Maria Imbaro (Chieti), 66030, Italy

    Stefania Spanò, Claudia Cericola, Carmen Valente, Antonino Colanzi & Daniela Corda

  3. Division of Rheumatology, and Department of Medicine, Immunology and Allergy, Brigham and Women's Hospital, Harvard Medical School, Boston, 02115, MA, USA

    Victor W. Hsu

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Bonazzi, M., Spanò, S., Turacchio, G. et al. CtBP3/BARS drives membrane fission in dynamin-independent transport pathways. Nat Cell Biol 7, 570–580 (2005). https://doi.org/10.1038/ncb1260

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