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Next-generation DNA sequencing

Nature Biotechnology volume 26, pages 1135–1145 (2008)Cite this article

Abstract

DNA sequence represents a single format onto which a broad range of biological phenomena can be projected for high-throughput data collection. Over the past three years, massively parallel DNA sequencing platforms have become widely available, reducing the cost of DNA sequencing by over two orders of magnitude, and democratizing the field by putting the sequencing capacity of a major genome center in the hands of individual investigators. These new technologies are rapidly evolving, and near-term challenges include the development of robust protocols for generating sequencing libraries, building effective new approaches to data-analysis, and often a rethinking of experimental design. Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes, transcriptomes and interactomes to become inexpensive, routine and widespread, rather than requiring significant production-scale efforts.

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Figure 1: Work flow of conventional versus second-generation sequencing.
Figure 2: Clonal amplification of sequencing features.
Figure 3: Strategies for cyclic array sequencing.

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Acknowledgements

We thank G. Church and G. Porreca for helpful comments on the manuscript.

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Authors and Affiliations

  1. Department of Genome Sciences, University of Washington, Foege Building S-250, Box 355065, 1705 NE Pacific St., Seattle, 98195-5065, Washington, USA

    Jay Shendure

  2. Stanford Genome Technology Center and Division of Oncology, Dept. of Medicine, Stanford University School of Medicine, CCSR 3215, 269 Campus Drive, Stanford, 94305, California, USA

    Hanlee Ji

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Correspondence to Jay Shendure or Hanlee Ji.

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J.S. has patents pending in the field of next-generation sequencing technology.

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Shendure, J., Ji, H. Next-generation DNA sequencing. Nat Biotechnol 26, 1135–1145 (2008). https://doi.org/10.1038/nbt1486

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