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Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice

Nature volume 377, pages 539–544 (1995)Cite this article

Abstract

THE intracellular protein tyrosine kinase FAK (focal adhesion kinase) was originally identified by its high level of tyrosine phosphorylation in v-src-transformed cells1á¤-4. FAK is also highly phosphorylated during early development5,6. In cultured cells it is localized to focal adhesion contacts and becomes phosphorylated and activated in response to integrin-mediated binding of cells to the extracellular matrix, suggesting an important role in cell adhesion and/or migration. We have generated FAK-deficient mice by gene targeting to examine the role of FAK during development. Mutant embryos displayed a general defect of mesoderm development, and cells from these embryos had reduced mobilityin vitro. Surprisingly, the number of focal adhesions was increased in FAK-deficient cells, suggesting that FAK may be involved in the turnover of focal adhesion contacts during cell migration.

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Authors and Affiliations

  1. Department of Morphogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, 2-2-1 Honjo, Kumamoto, 860, Japan

    Duško llić, Yasuhide Furuta, Satoshi Kanazawa, Naoki Takeda & Shinichi Aizawa

  2. Department of Oncology, Institute of Medical Science, Tokyo University, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108, Japan

    Duško llić, Jiro Fujimoto & Tadashi Yamamoto

  3. Department of Neurochemistry and Neuropharmacology, Biomedical Research Centre, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, 565, Japan

    Kenji Sobue

  4. Department of Pathology, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, 565, Japan

    Shintaro Nomura

  5. Division of Protein Metabolism, Institute for Protein Research, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka, 565, Japan

    Masato Okada

  6. Mammalian Development Laboratory, National Institute of Genetics, 1111 Yata, Mishima, 411, Japan

    Norio Nakatsuji

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  1. Duško llić
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llić, D., Furuta, Y., Kanazawa, S. et al. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature 377, 539–544 (1995). https://doi.org/10.1038/377539a0

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