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Signalling downstream of activated mammalian Notch

Nature volume 377, pages 355–358 (1995)Cite this article

Abstract

NOTCH belongs to a family of transmembrane proteins that are widely conserved from flies to vertebrates and are thought to be involved in cell-fate decisions. In Drosophila, the Suppressor of hairless (Su(H)) gene1,2 and genes of the Enhancer of split (E(Spl)) complex, which encode proteins of the basic helix–loop–helix type3,4 have been implicated in the Notch signalling pathway. Mammalian homologues of E(Spl), such as the mouse Hairy enhancer of split (HES-1 )5, have been isolated. Both HES-1 and the intracellular domain of murine Notch (mNotch) are able to block MyoD-induced myogenesis5-7. Here we show that activated forms of mNotch associate with the human analogue of Su(H), KBF2/RBP-JK (refs 8, 9) and act as transcriptional activators through the KBF2-binding sites of the HES-1 promoter.

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Authors and Affiliations

  1. Division of Dermatology and the Department of Molecular Biology and Pharmacology, Washington University, Box 8123, 4940 Parkview Place, St Louis, Missouri, 63110, USA

    Sophie Jarriault, Christel Brou, Frédérique Logeat & Alain Israel

  2. Unite de Biologie Moleculaire de L'Expression Genique, URA 1149 CNRS, Institut Pasteur, 28 rue du Dr Roux, 75724, Paris, Cedex 15, France

    Eric H. Schroeter & Raphael Kopan

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  1. Sophie Jarriault
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  2. Christel Brou
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  4. Eric H. Schroeter
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  5. Raphael Kopan
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  6. Alain Israel
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Jarriault, S., Brou, C., Logeat, F. et al. Signalling downstream of activated mammalian Notch. Nature 377, 355–358 (1995). https://doi.org/10.1038/377355a0

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