Abstract
The 1.8 Å crystal structure of transducin α-GDP, when compared to that of the activated complex with GTP-γS, reveals the nature of the conformational changes that occur on activation of a heterotrimeric G-protein α-subunit. Structural changes initiated by direct contacts with the terminal phosphate of GTP propagate to regions that have been implicated in effector activation. The changes are distinct from those observed in other members of the GTPase superfamily.
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References
Hepler, J. R. & Gilman, A. G. Trends Biochem. Sci. 17, 383–387 (1992).
Simon, M. I., Strathmann, M. P. & Gautam, N. Science 252, 802–808 (1991).
Conklin, B. R. & Bourne, H. R. Cell 73, 631–641 (1993).
Hurley, J. B. J. Bioenerg. Biomembranes 24, 219–226 (1992).
Pfister, C. et al. Cell Sig. 5, 235–241 (1993).
Hargrave, P. A. & Hamm, H. E. in Molecular Pharmacoloy of Cell Regulation Vol. 3 (eds Sibley, D. R. & Houslay, M. D.) 25–67 (Wiley, New York, 1994).
Bourne, H. R., Sanders, D. A. & McCormick, F. Nature 348, 125–132 (1990).
Pai, E. F. et al. Nature 341, 209–214 (1989).
Brunger, A. T. et al. Proc. natn. Acad. Sci. U.S.A. 87, 4849–4853 (1990).
Tong, L. et al. J. molec. Biol. 217, 503–516 (1991).
Jurnak, F. Science 230, 32–36 (1985).
Berchtold, H. et al. Nature 365, 126–132 (1993).
Noel, J. P., Hamm, H. E. & Sigler, P. B. Nature 366, 654–663 (1993).
Mazzoni, M. R., Malinski, J. A. & Hamm, H. E. J. biol. Chem. 266, 14072–14081 (1991).
Sternweis, P. C. & Gilman, A. C. Proc. natn. Acad. Sci. U.S.A. 79, 4888–4891 (1982).
Fung, B. K.-K. & Nash, C. R. J. biol. Chem. 258, 10503–10510 (1983).
Mazzoni, M. R. & Hamm, H. E. J. J. Prot. Chem. 12, 215–221 (1993).
Higashijima, T. et al. J. biol. Chem. 262, 752–756 (1987).
Faurobert, E. et al. EMBO J. 12, 4191–4198 (1993).
Miller, R. T. et al. Nature 267, 712–715 (1988).
Lee, E., Taussig, R. & Gilman, A. G. J. biol. Chem. 267, 1212–1218 (1992).
Clark, K. L., Dignard, D., Thomas, D. Y. & Whiteway, M. Molec. cell. Biol. 13, 1–8 (1993).
Whiteway, M. et al. Molec. cell. Biol. (in the press).
Thomas, T. C., Schmidt, C. J. & Neer, E. J. Proc. natn. Acad. Sci. U.S.A. 90, 10295–10298 (1993).
Rarick, H. M., Artemyev, N. O. & Hamm, H. E. Science 256, 1031–1033 (1992).
Berlot, C. H. & Bourne, H. R. Cell 68, 911–922 (1992).
Stryer, L., Hurley, J. B. & Fung, B. K.-K. Meth. Enzym. 96, 617–627 (1983).
Brunger, A. T. Acta. crystallogr. A46, 46–57 (1990).
Brunger, A. T. X-PLOR Version 3.1 Manual (Yale University, 1993).
Jones, T. A. et al. Acta crystallogr. A47, 110–119 (1991).
Jones, T. A. J. J. appl. Crystallogr. 11, 268–276 (1978).
Yatsunami, K. & Khorana, H. G. Proc. natn. Acad. Sci. U.S.A. 82, 4316–4320 (1985).
Robishaw, J. D. et al. Proc. natn. Acad. Sci. U.S.A. 83, 1251–1255 (1986).
Nukada, T. et al. FEBS Lett. 197, 305–308 (1986).
Van Meurs, K. P. et al. Proc. natn. Acad. Sci. U.S.A. 84, 3107–3111 (1987).
Strathmann, M. & Simon, M. I. Proc. natn. Acad. Sci. U.S.A. 87, 9113–9117 (1990).
Fong, H. K. W., Yoshimoto, K. K., Eversole-Cire, P. & Simon, M. I. Proc. natn. Acad. Sci. U.S.A. 85, 3066–3070 (1988).
Nakafuku, M., Itoh, H., Nakamura, S. & Kaziro, Y. Proc. natn. Acad. Sci. U.S.A. 84, 2140–2144 (1987).
Capon, D. J., Chen, E. Y., Levinson, A. D., Seeburg, P. H. & Goeddel, D. Nature 302, 33–37 (1983).
Laursen, R. A., L'Italien, J. J., Nagarkatti, S. & Miller, D. L. J. biol. Chem. 256, 8102–8109 (1981).
Fawzi, A. B. & Northrup, J. K. Biochemistry 29, 3804–3812 (1990).
Higashijima, T. et al. J. biol. Chem. 262, 762–766 (1987).
John, J., Frech, M. & Wittinghofer, A. J. biol. Chem. 263, 11792–11799 (1988).
Kjeldgaard, M. & Nybord, J. J. molec. Biol. 223, 721–742 (1992).
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Lambright, D., Noel, J., Hamm, H. et al. Structural determinants for activation of the α-subunit of a heterotrimeric G protein. Nature 369, 621–628 (1994). https://doi.org/10.1038/369621a0
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DOI: https://doi.org/10.1038/369621a0