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Neurotrophins promote motor neuron survival and are present in embryonic limb bud

Nature volume 363, pages 266–270 (1993)Cite this article

Abstract

Embryonic spinal motor neurons are thought to depend for survival on unidentified factors secreted both by their peripheral targets and by cells within the central nervous system1. The neurotrophins are a family of polypeptides required for survival of discrete central and peripheral neuronal populations in vivo and in vitro2,3. In spite of their ability to reduce motor neuron death in vivo4–6, the known neurotrophins have been thought to be without direct effect on motor neurons7–10. Here we show that picomolar concentrations of three of them, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-5, can prevent the death of cultured embryonic rat spinal motor neurons. Furthermore, messenger RNA coding for neurotrophins is present at appropriate stages in spinal cord and limb bud, and mRNA for their receptors is found in motor neurons. These neurotrophins may therefore be physiological motor neuron growth factors.

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Authors and Affiliations

  1. Centre de Recherche de Biochimie Macromoléculaire, UPR 9008 du CNRS/U249 de I'lNSERM, BP. 5051, 34033, Montpellier, France

    Christopher E. Henderson, William Camu, Clément Mettling & Annie Gouin

  2. Department of Cell Analysis and § Department of Neuroscience, Genentech Inc., South San Francisco, California, 94080, USA

    Kristian Poulsen, Mona Karihaloo, Janette Ruilamas & Tony Evans

  3. Department of Neuroscience, Genentech Inc., South San Francisco, California, 94080, USA

    Mark P. Armanini, Lucy Berkemeier, Heidi S. Phillips & Arnon Rosenthal

  4. Department of Physiology, UMDS (St Thomas' Campus), Lambeth Palace Road, London, SE1 7EH, UK

    Stephen B. McMahon

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  1. Christopher E. Henderson
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  2. William Camu
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  3. Clément Mettling
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  4. Annie Gouin
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Henderson, C., Camu, W., Mettling, C. et al. Neurotrophins promote motor neuron survival and are present in embryonic limb bud. Nature 363, 266–270 (1993). https://doi.org/10.1038/363266a0

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