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Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides

Nature volume 358, pages 646–653 (1992)Cite this article

Abstract

Three-dimensional structures of complexes of the SH2 domain of the v-src oncogene product with two phosphotyrosyl peptides have been determined by X-ray crystallography at resolutions of 1.5 and 2.0 Å, respectively. A central antiparallel β-sheet in the structure is flanked by two α-helices, with peptide binding mediated by the sheet, intervening loops and one of the helices. The specific recognition of phosphotyrosine involves amino–aromatic interactions between lysine and arginine side chains and the ring system in addition to hydrogen-bonding interactions with the phosphate.

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Authors and Affiliations

  1. The Rockefeller University, 1230 York Avenue, New York, 10021, USA

    Gabriel Waksman, Dorothea Kominos, Scott C. Robertson, Nalin Pant, David Baltimore, Raymond B. Birge, David Cowburn, Hidesaburo Hanafusa, Bruce J. Mayer, Michael Overduin, Marilyn D. Resh, Carlos B. Rios, Lauren Silverman & John Kuriyan

  2. Howard Hughes Medical Institute, 1230 York Avenue, New York, 10021, USA

    Scott C. Robertson & John Kuriyan

  3. Department of Cell Biology and Genetics, Sloan Kettering Institute for Cancer Research, 1275 York Avenue, New York, 10021, USA

    Marilyn D. Resh & Lauren Silverman

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Waksman, G., Kominos, D., Robertson, S. et al. Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides. Nature 358, 646–653 (1992). https://doi.org/10.1038/358646a0

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